br Conclusion br In summary IL GFE exhibit antiproliferative e
In summary, IL-GFE exhibit antiproliferative eﬀects against MCF-7 breast cancer cell lines by inducing loss of cell viability via apoptosis, morphology changes, and cell cycle arrest at the G0/G1 phase. This inhibition was selective to the growth of MCF-7 cells without any eﬀect on nontumorigenic VERO cells, suggesting that IL-GFE possesses se-lective antitumor properties toward cancer cells. It also showed their potentiality as an anticancer agent when compared with Taxol as a positive control. Moreover, IL-GFE treatment has inhibited breast cancer MCF-7 cell growth by reducing the number of cell generations and increasing the doubling time compared with the control. These data suggest that the IL-GFE can be developed as a novel mechanism-based supplement agent for the cure and prevention of breast cancer. Further investigations are necessary to elucidate the mechanisms underlying
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the therapeutic eﬀects of the fruit extract as well as the determination of the active compounds responsible for cytotoxicity.
Conflicts of interest
There is no conflict of interest in this work.
The authors are grateful to the International Islamic University Malaysia, for providing the P-RIGS grant and laboratory facilities to carry out the research.
Appendix A. Supplementary data
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Antiproliferative and apoptotic activities of 8-prenylnaringenin against