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  • br Conclusion br In summary IL GFE exhibit antiproliferative e

    2022-05-22


    5. Conclusion
    In summary, IL-GFE exhibit antiproliferative effects against MCF-7 breast cancer cell lines by inducing loss of cell viability via apoptosis, morphology changes, and cell cycle arrest at the G0/G1 phase. This inhibition was selective to the growth of MCF-7 cells without any effect on nontumorigenic VERO cells, suggesting that IL-GFE possesses se-lective antitumor properties toward cancer cells. It also showed their potentiality as an anticancer agent when compared with Taxol as a positive control. Moreover, IL-GFE treatment has inhibited breast cancer MCF-7 cell growth by reducing the number of cell generations and increasing the doubling time compared with the control. These data suggest that the IL-GFE can be developed as a novel mechanism-based supplement agent for the cure and prevention of breast cancer. Further investigations are necessary to elucidate the mechanisms underlying
    D. Daddiouaissa, et al.
    the therapeutic effects of the fruit extract as well as the determination of the active compounds responsible for cytotoxicity.
    Conflicts of interest
    There is no conflict of interest in this work.
    Acknowledgment
    The authors are grateful to the International Islamic University Malaysia, for providing the P-RIGS grant and laboratory facilities to carry out the research.
    Appendix A. Supplementary data
    References
    Agu, K.C., Okolie, N.P., Falodun, A., Engel-Lutz, N., 2018. In vitro anticancer assessments of Annona muricata fractions and in vitro antioxidant profile of fractions and isolated acetogenin (15-acetyl guanacone). Journal of Cancer Research and Practice 5 (2), 53–66.
    Bhan, M., Satija, S., Garg, C., Dureja, H., Garg, M., 2017. A novel approach towards green extraction for glycyrrhitinic SU5416 by ionic liquid based microwave assisted extraction and optimization through response surface methodology. Phcog. J. 9 (6). Boik, J., 2001. Natural Compounds in Cancer Therapy.
    Gajalakshmi, S., Vijayalakshmi, S., Devi SU5416 Rajeswari, V., 2012. Phytochemical and phar-macological properties of Annona muricata: a review. Int. J. Pharm. Pharm. Sci. 4
    Gavamukulya, Y., Abou-Elella, F., Wamunyokoli, F., AEl-Shemy, H., 2014. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific journal of tropical medicine 7, S355–S363.
    George, V.C., Kumar, D., Rajkumar, V., Suresh, P., Kumar, R.A., 2012. Quantitative as-sessment of the relative antineoplastic potential of the n-butanolic leaf extract of Annona muricata Linn. in normal and immortalized human cell lines. Asian Pac. J. Cancer Prev. APJCP 13 (2), 699–704.
    from some selected communities in Ghana by instrumental neutron activation ana-lysis. Elixir Food Sci 41, 5671–5675. Halliwell, B., 2012. Free radicals and antioxidants: updating a personal view. Nutr. Rev.
    Magadi, V.P., Ravi, V., Arpitha, A., 2015. Evaluation of cytotoxicity of aqueous extract of Graviola leaves on squamous cell carcinoma cell-25 cell lines by 3-(4, 5-di-methylthiazol-2-Yl)-2, 5-diphenyltetrazolium bromide assay and determination of percentage of cell inhibition at G2M phase of cell cycle by flow cytometry: an in vitro study. Contemp. Clin. Dent. 6 (4), 529.
    Zhang, Y., Liu, Z., Li, Y., Chi, R., 2014. Optimization of ionic liquid-based microwave-assisted extraction of isoflavones from Radix puerariae by response surface metho-dology. Separ. Purif. Technol. 129, 71–79.
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    Antiproliferative and apoptotic activities of 8-prenylnaringenin against